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Leica Cell DIVE - a robust approach to whole tissue imaging

• Built for Life Science Researchers: a user friendly yet highly stable system designed for studying tissues in detail.
• Whole tissue imaging: uses a widefield microscope - especially useful for understanding where cells are located and how they interact in tissue samples (spatial biology).
• Fully automated: works with the  to automatically stain, image, and process multiple slides —even runs 24/7.

How it works

Traditional methods:

• All targets are labelled and imaged in one go
• Multiplexing can be difficult because the fluorescent dyes can overlap and interfere with each other.
• Requires technical expertise to separate similar dyes

Cell Dive’s approach:

• Labels and images 4 targets at a time, multiplexing is achieved via multiple rounds of labelling and imaging.
• This avoids overlap and makes it easier to study many different markers clearly.
• Simple concept thus no expertise required

Advantages of this microscope

• Easy to use: you don’t need to be a microscopy expert to get great results.
• Hands-free operation: the system focuses and runs on its own — no need for constant supervision.
• High capacity: can analyse over 60 different markers in a single tissue sample — helping researchers understand complex cell types and functions.
• Clear images: produces high-quality, full-tissue images that look like traditional H&E stains.
• Timesaving: the robot handles the work, so you don’t have to stand by the microscope.
• Reliable results: built-in calibration ensures consistent and accurate imaging every time.

Location

Room 304B, Imperial Centre for Translational and Experimental Medicine - ICTEM
Hammersmith Campus
Du Cane Road, London, W12 0NN

Facilities available in room 304B:

Quickstart guides

Quickstart guide - HMI1 - Leica Cell DIVE Multiplex Imager

Quick start guide - BioAssemblyBot 200 (BAB200)

Objectives for imaging

• 2x CFI Plan Apo Lambda (general overview)
• 10x CFI Plan Apo Lambda (region of interest selection)
• 20x CFI S Plan Fluor LWD (final imaging)

Light source and emission filter

Validated antibodies

Technical notes

Leica webinars

Offline software

• HALO
• FIJI
• Qu Path

Visit the FILM pages on image analysis software.