BibTex format
@article{Bennison:2026:10.1038/s44318-026-00830-z,
author = {Bennison, DJ and Chaudhary, I and Chaudhuri, D and Wong, JCN and Punwatkar, A and Biswas, P and Stephenson, M and Zhong, Q and Kallemeijn, WW and Guenot, M and Koigi, S and Papp, D and Thomas, JP and Dixit, D and Korcsmaros, T and Talman, AM and Frickel, E-M and Tate, EW and Visweswariah, SS and Frankel, G and Shenoy, AR},
doi = {10.1038/s44318-026-00830-z},
journal = {EMBO Journal},
title = {GBP1 recruitment to actin-rich pedestals of extracellular Gram-negative bacteria promotes pyroptosis},
url = {http://dx.doi.org/10.1038/s44318-026-00830-z},
year = {2026}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - The IFNγ-induced GTPase guanylate-binding protein 1 (GBP1) binds to lipopolysaccharide (LPS) on cytosolic gram-negative bacteria and promotes pyroptosis via the recruitment and activation of caspase-4 on the bacterial outer membrane. Enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC, respectively) are extracellular pathogens that adhere to host cells and stimulate dense actin polymerisation underneath their attachment sites, generating structures described as actin-rich pedestals. Here, we show that GBP1 traffics to actin-rich pedestals in human cells infected with EPEC or EHEC in vitro and mouse colonocytes infected with the EPEC-like murine pathogen Citrobacter rodentium in vivo. GBP1 promotes caspase-4 recruitment to actin-rich pedestals, leading to pyroptosis and IL-18 release. GBP1 mutants defective in LPS coatomer formation also localise to EPEC pedestals. A novel assay that mimics pathogenic effector activity reveals GBP1 recruitment to sterile actin polymerisation sites. We conclude that cytosolic GBP1 is mobilised to sites of pathogen-induced actin remodelling independently of LPS. Our study establishes that GBP1 not only operates as a pattern-recognition receptor but also orchestrates effector-triggered immunity against pathogens that hijack the actin cytoskeleton.
AU - Bennison,DJ
AU - Chaudhary,I
AU - Chaudhuri,D
AU - Wong,JCN
AU - Punwatkar,A
AU - Biswas,P
AU - Stephenson,M
AU - Zhong,Q
AU - Kallemeijn,WW
AU - Guenot,M
AU - Koigi,S
AU - Papp,D
AU - Thomas,JP
AU - Dixit,D
AU - Korcsmaros,T
AU - Talman,AM
AU - Frickel,E-M
AU - Tate,EW
AU - Visweswariah,SS
AU - Frankel,G
AU - Shenoy,AR
DO - 10.1038/s44318-026-00830-z
PY - 2026///
SN - 0261-4189
TI - GBP1 recruitment to actin-rich pedestals of extracellular Gram-negative bacteria promotes pyroptosis
T2 - EMBO Journal
UR - http://dx.doi.org/10.1038/s44318-026-00830-z
ER -