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  • Journal article
    Hare S, Vos AM, Clayton RF, Thuring JW, Cummings MD, Cherepanov Pet al., 2010,

    , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 20057-20062, ISSN: 0027-8424
    • Cite
    • Citations: 269
  • Journal article
    Lu D, Fillet S, Meng C, Alguel Y, Kloppsteck P, Bergeron J, Krell T, Gallegos M-T, Ramos J, Zhang Xet al., 2010,

    , GENES & DEVELOPMENT, Vol: 24, Pages: 2556-2565, ISSN: 0890-9369
    • Cite
    • Citations: 33
  • Journal article
    Maertens GN, Hare S, Cherepanov P, 2010,

    , Nature, Vol: 468, Pages: 326-U217, ISSN: 0028-0836

    To establish productive infection, a retrovirus must insert a DNA replica of its genome into host cell chromosomal DNA1,2. This process is operated by the intasome, a nucleoprotein complex composed of an integrase tetramer (IN) assembled on the viral DNA ends3,4. The intasome engages chromosomal DNA within a target capture complex to carry out strand transfer, irreversibly joining the viral and cellular DNA molecules. Although several intasome/transpososome structures from the DDE(D) recombinase superfamily have been reported4,5,6, the mechanics of target DNA capture and strand transfer by these enzymes remained unclear. Here we report crystal structures of the intasome from prototype foamy virus in complex with target DNA, elucidating the pre-integration target DNA capture and post-catalytic strand transfer intermediates of the retroviral integration process. The cleft between IN dimers within the intasome accommodates chromosomal DNA in a severely bent conformation, allowing widely spaced IN active sites to access the scissile phosphodiester bonds. Our results resolve the structural basis for retroviral DNA integration and provide a framework for the design of INs with altered target sequences.
  • Journal article
    Schroeder GN, Petty NK, Mousnier A, Harding CR, Vogrin AJ, Wee B, Fry NK, Harrison TG, Newton HJ, Thomson NR, Beatson SA, Dougan G, Hartland EL, Frankel Get al., 2010,

    , JOURNAL OF BACTERIOLOGY, Vol: 192, Pages: 6001-6016, ISSN: 0021-9193
    • Cite
    • Citations: 94
  • Journal article
    Choudhury HG, Beis K, 2010,

    , ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 66, Pages: 1496-1499
  • Journal article
    Ramboarina S, Garnett JA, Zhou M, Li Y, Peng Z, Taylor JD, Lee W-C, Bodey A, Murray JW, Alguel Y, Bergeron J, Bardiaux B, Sawyer E, Isaacson R, Tagliaferri C, Cota E, Nilges M, Simpson P, Ruiz T, Wu H, Matthews Set al., 2010,

    , JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 32446-32457, ISSN: 0021-9258
    • Cite
    • Citations: 45
  • Journal article
    Ryan AJ, Ghuman J, Zunszain PA, Chung C-W, Curry Set al., 2010,

    , Journal of Structural Biology, Vol: 174, Pages: 84-91, ISSN: 1095-8657
  • Journal article
    Vossenkaemper A, Marches O, Fairclough PD, Warnes G, Stagg AJ, Lindsay JO, Evans PC, Luong LA, Croft NM, Naik S, Frankel G, MacDonald TTet al., 2010,

    , JOURNAL OF IMMUNOLOGY, Vol: 185, Pages: 4118-4127, ISSN: 0022-1767
    • Cite
    • Citations: 68
  • Journal article
    Karatsa-Dodgson M, Woermann ME, Gruendling A, 2010,

    , JOURNAL OF BACTERIOLOGY, Vol: 192, Pages: 5341-5349, ISSN: 0021-9193
    • Cite
    • Citations: 33
  • Journal article
    Watson KG, Holden DW, 2010,

    , CELLULAR MICROBIOLOGY, Vol: 12, Pages: 1389-1397, ISSN: 1462-5814
    • Cite
    • Citations: 110
  • Journal article
    Yan R, Simpson PJ, Matthews SJ, Cota Eet al., 2010,

    , BIOMOLECULAR NMR ASSIGNMENTS, Vol: 4, Pages: 187-190, ISSN: 1874-2718
  • Journal article
    Michoux F, Takasaka K, Boehm M, Nixon PJ, Murray JWet al., 2010,

    , BIOCHEMISTRY, Vol: 49, Pages: 7411-7413, ISSN: 0006-2960
    • Cite
    • Citations: 45
  • Journal article
    Krishnan L, Li X, Naraharisetty HL, Hare S, Cherepanov P, Engelman Aet al., 2010,

    , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 15910-15915, ISSN: 0027-8424
    • Cite
    • Citations: 175
  • Journal article
    Bush M, Ghosh T, Tucker N, Zhang X, Dixon Ret al., 2010,

    , MOLECULAR MICROBIOLOGY, Vol: 77, Pages: 1278-1288, ISSN: 0950-382X
    • Cite
    • Citations: 18
  • Journal article
    Berger CN, Sodha SV, Shaw RK, Griffin PM, Pink D, Hand P, Frankel Get al., 2010,

    , ENVIRONMENTAL MICROBIOLOGY, Vol: 12, Pages: 2385-2397, ISSN: 1462-2912
    • Cite
    • Citations: 663
  • Journal article
    McClelland A, Demidov A, Benabbas A, Sun Y, Venugopal K, Sage T, van Thor J, Champion Pet al., 2010,

    Investigation of excited state proton transfer in green fluorescent protein

    , ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol: 240, ISSN: 0065-7727
  • Journal article
    Sweeney TR, Cisnetto V, Bose D, Bailey M, Wilson JR, Zhang X, Belsham GJ, Curry Set al., 2010,

    , JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 24347-24359
    • Cite
    • Citations: 59
  • Journal article
    Dumont A, Boucrot E, Drevensek S, Daire V, Gorvel J-P, Poues C, Holden DW, Meresse Set al., 2010,

    , TRAFFIC, Vol: 11, Pages: 899-911, ISSN: 1398-9219
    • Cite
    • Citations: 90
  • Journal article
    Munera D, Crepin VF, Marches O, Frankel Get al., 2010,

    , JOURNAL OF BACTERIOLOGY, Vol: 192, Pages: 3534-3539, ISSN: 0021-9193
    • Cite
    • Citations: 30
  • Journal article
    Sadovskaya I, Vinogradov E, Li J, Hachani A, Kowalska K, Filloux Aet al., 2010,

    , GLYCOBIOLOGY, Vol: 20, Pages: 895-904, ISSN: 0959-6658
    • Cite
    • Citations: 88
  • Journal article
    Nixon PJ, Michoux F, Yu J, Boehm M, Komenda Jet al., 2010,

    , ANNALS OF BOTANY, Vol: 106, Pages: 1-16, ISSN: 0305-7364
    • Cite
    • Citations: 443
  • Journal article
    Salacha R, Kovacic F, Brochier-Armanet C, Wilhelm S, Tommassen J, Filloux A, Voulhoux R, Bleves Set al., 2010,

    , ENVIRONMENTAL MICROBIOLOGY, Vol: 12, Pages: 1498-1512, ISSN: 1462-2912
    • Cite
    • Citations: 75
  • Journal article
    Kertis F, Snyder J, Govada L, Khurshid S, Chayen N, Erlebacher Jet al., 2010,

    , JOM, Vol: 62, Pages: 50-56, ISSN: 1047-4838
    • Cite
    • Citations: 84
  • Journal article
    Robinson KS, Mousnier A, Hemrajani C, Fairweather N, Berger CN, Frankel Get al., 2010,

    , MICROBIOLOGY-SGM, Vol: 156, Pages: 1815-1823, ISSN: 1350-0872
    • Cite
    • Citations: 25
  • Journal article
    Yu X-J, McGourty K, Liu M, Unsworth KE, Holden DWet al., 2010,

    , SCIENCE, Vol: 328, Pages: 1040-1043, ISSN: 0036-8075
    • Cite
    • Citations: 152
  • Journal article
    Chen S, McDowall A, Dobro MJ, Briegel A, Ladinsky M, Shi J, Tocheva EI, Beeby M, Pilhofer M, Ding HJ, Li Z, Gan L, Morris DM, Jensen GJet al., 2010,

    Electron cryotomography of bacterial cells

    , Jove-Journal of Visualized Experiments, Vol: 39, ISSN: 1940-087X

    While much is already known about the basic metabolism of bacterial cells, many fundamental questions are still surprisingly unanswered,including for instance how they generate and maintain specific cell shapes, establish polarity, segregate their genomes, and divide. In orderto understand these phenomena, imaging technologies are needed that bridge the resolution gap between fluorescence light microscopy andhigher-resolution methods such as X-ray crystallography and NMR spectroscopy.Electron cryotomography (ECT) is an emerging technology that does just this, allowing the ultrastructure of cells to be visualized in a near-nativestate, in three dimensions (3D), with "macromolecular" resolution (~4nm).1, 2 In ECT, cells are imaged in a vitreous, "frozen-hydrated" state ina cryo transmission electron microscope (cryoTEM) at low temperature (< -180°C). For slender cells (up to ~500 nm in thickness3), intact cellsare plunge-frozen within media across EM grids in cryogens such as ethane or ethane/propane mixtures. Thicker cells and biofilms can alsobe imaged in a vitreous state by first "high-pressure freezing" and then, "cryo-sectioning" them. A series of two-dimensional projection imagesare then collected through the sample as it is incrementally tilted along one or two axes. A three-dimensional reconstruction, or "tomogram" canthen be calculated from the images. While ECT requires expensive instrumentation, in recent years, it has been used in a few labs to reveal thestructures of various external appendages, the structures of different cell envelopes, the positions and structures of cytoskeletal filaments, andthe locations and architectures of large macromolecular assemblies such as flagellar motors, internal compartments and chemoreceptor arrays.1,2In this video article we illustrate how to image cells with ECT, including the processes of sample preparation, data collection, tomogramreconstruction, and interpre
  • Journal article
    Klopfleisch C, Minh LQ, Giesow K, Curry S, Keil GMet al., 2010,

    , ARCHIVES OF VIROLOGY, Vol: 155, Pages: 723-731, ISSN: 0304-8608
  • Journal article
    Cherepanov P, 2010,

    , EMBO REPORTS, Vol: 11, Pages: 328-328, ISSN: 1469-221X
    • Cite
    • Citations: 20
  • Journal article
    Komenda J, Knoppova J, Krynicka V, Nixon PJ, Tichy Met al., 2010,

    , BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Vol: 1797, Pages: 566-575, ISSN: 0005-2728
    • Cite
    • Citations: 40
  • Journal article
    Sciara G, Bebeacua C, Bron P, Tremblay D, Ortiz-Lombardia M, Lichiere J, van Heel M, Campanacci V, Moineau S, Cambillau Cet al., 2010,

    , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 6852-6857, ISSN: 0027-8424
    • Cite
    • Citations: 141

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